Vincent F, Feuerstein C, Gavend M, Faure J
Clin Chim Acta. 1979 May 2;93(3):391-9. doi: 10.1016/0009-8981(79)90290-0.
A very easy, reliable and specific gas-chromatographic method for identification and dosage of 11 barbiturates in plasma is presented. Methylene unit values determined on two types of column (one polar, one non-polar) allows very accurate identification with a minimum risk of error due to fluctuations in operating conditions. Sensitivity of the method is 0.5 microgram/ml plasma with a flame ionization detector. Derivatization procedure is complete, without any degradation phenomenon as tested by mass spectrometry. Such a method can be easily used as routine procedure for toxicological or pharmacological applications.
本文介绍了一种非常简便、可靠且特异的气相色谱法，用于血浆中11种巴比妥类药物的鉴定和定量。在两种类型的色谱柱（一种极性柱，一种非极性柱）上测定的亚甲基单位值能够实现非常准确的鉴定，因操作条件波动导致的误差风险最小。使用火焰离子化检测器时，该方法的灵敏度为0.5微克/毫升血浆。衍生化过程完整，经质谱检测无任何降解现象。这种方法可轻松用作毒理学或药理学应用的常规方法。
