Konomi K, Yamamoto H, Nishimura M
Jpn J Surg. 1974 Mar;4(1):48-55. doi: 10.1007/BF02471381.
Human gallbladder bile was separated by salting-in column chromatography using silica gel ammonium sulfate system. The second fraction containing peptides was found to vary in cholelithiasis in relation to Fraction 1. To determine the structure of the peptides, bovine gallbladder bile was fractionated by salting out method with ammonium sulfate. Four peptides were isolated and their amino acid sequences were determined to be: (see article).
人胆囊胆汁通过硅胶硫酸铵体系的盐析柱色谱法进行分离。发现含有肽的第二部分在胆结石方面相对于第一部分有所不同。为了确定这些肽的结构,用硫酸铵盐析法对牛胆囊胆汁进行分级分离。分离出了四种肽,并确定它们的氨基酸序列为:(见文章)。