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[气相色谱法测定血浆卵磷脂胆固醇酰基转移酶(LCAT)活性]

[Gas-chromatographic method for determination of plasma lecithin-cholesterol acyl transferase (LCAT) activity].

作者信息

Ciampi G, Messeri G

出版信息

Quad Sclavo Diagn. 1974 Dec;10(4):536-49.

PMID:4460077
Abstract

A method is described for the determination of plasma lecythin-cholesterol acyl-transferase activity (LCAT), based on the measuring ot the free cholesterol concentration decrement in incubated plasma, with the aid of gas-liquid chromatography. Precision evaluation showed a sufficient reliability of the method, which is considered to be proposable for practical puopose. After verifying normal values in a group of healthy adults, plasma LCAT reduction in liver diseases, specially in the chronic hepatic disease, was confirmed.

摘要

本文描述了一种基于气液色谱法测量孵育血浆中游离胆固醇浓度降低来测定血浆卵磷脂胆固醇酰基转移酶活性(LCAT)的方法。精密度评估表明该方法具有足够的可靠性,被认为可用于实际目的。在一组健康成年人中验证正常值后,证实了肝脏疾病,特别是慢性肝病患者血浆LCAT降低。

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