硫醇阻断试剂与磷酸乙酰转移酶催化作用，以及吸附分子的保护作用评估。
Thiol-blocking reagents and phosphate acetyltransferase catalysis, and the assessment of protection by adsorbed molecules.
作者信息
Kyrtopoulos S A, Satchell D P
出版信息
Biochem J. 1974 Sep;141(3):905-7. doi: 10.1042/bj1410905.
Abstract
Phosphate acetyltransferase (EC 2.3.1.8) is shown to be unusually sensitive to thiol-blocking reagents, and one or more thiol groups probably catalyse the surface reaction. A novel method for assessing protection by adsorbed species is described. The adsorbed substrate molecules acetyl-CoA and phosphate are found to provide substantial protection against thiol-blocking reagents.
摘要
磷酸乙酰转移酶（EC 2.3.1.8）被证明对硫醇阻断剂异常敏感，可能有一个或多个硫醇基团催化表面反应。本文描述了一种评估吸附物质保护作用的新方法。研究发现，吸附的底物分子乙酰辅酶A和磷酸盐能为硫醇阻断剂提供显著的保护作用。
相似文献
1
Thiol-blocking reagents and phosphate acetyltransferase catalysis, and the assessment of protection by adsorbed molecules.硫醇阻断试剂与磷酸乙酰转移酶催化作用，以及吸附分子的保护作用评估。
Biochem J. 1974 Sep;141(3):905-7. doi: 10.1042/bj1410905.
2
Choline acetyltransferase. Inhibition by thiol reagents.胆碱乙酰转移酶。硫醇试剂的抑制作用。
J Biol Chem. 1974 Apr 10;249(7):2156-9.
3
Inhibition of choline acetyltransferase from bovine caudate nucleus by sulfhydryl reagents and reactivation of the inhibited enzyme.巯基试剂对牛尾状核胆碱乙酰转移酶的抑制作用及对被抑制酶的复活作用
Biochem Pharmacol. 1970 Aug;19(8):2509-16. doi: 10.1016/0006-2952(70)90277-7.
4
Derivatization of the interface cysteine of triosephosphate isomerase from Trypanosoma brucei and Trypanosoma cruzi as probe of the interrelationship between the catalytic sites and the dimer interface.将布氏锥虫和克氏锥虫磷酸丙糖异构酶的界面半胱氨酸进行衍生化，以此作为催化位点与二聚体界面之间相互关系的探针。
Biochemistry. 1999 Mar 30;38(13):4114-20. doi: 10.1021/bi982425s.
5
Inhibition of leucine decarboxylase by thiol-binding reagents.硫醇结合试剂对亮氨酸脱羧酶的抑制作用。
Arch Biochem Biophys. 1962 Feb;96:360-70. doi: 10.1016/0003-9861(62)90421-6.
6
Active-site-directed inhibition of carnitine acetyltransferase.
Arch Biochem Biophys. 1983 Jan;220(1):193-9. doi: 10.1016/0003-9861(83)90400-9.
7
Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes.
Biochemistry. 1975 Feb 25;14(4):766-71. doi: 10.1021/bi00675a019.
8
9
Irreversible inhibition of phosphotransacetylase by S-dimethylarsino-CoA.
Biochim Biophys Acta. 1983 Nov 28;749(1):84-90. doi: 10.1016/0167-4838(83)90154-1.
10
Study of the Escherichia coli tRNA nucleotidyltransferase. Effect of inorganic ions and thiol blocking reagents on enzyme activity.
Biochim Biophys Acta. 1974 Aug 29;361(2):176-84.
引用本文的文献
1
Structural and functional studies suggest a catalytic mechanism for the phosphotransacetylase from Methanosarcina thermophila.结构和功能研究表明了嗜热甲烷八叠球菌磷酸转乙酰酶的催化机制。
J Bacteriol. 2006 Feb;188(3):1143-54. doi: 10.1128/JB.188.3.1143-1154.2006.
2
SH groups in the alpha-naphthyl acetate esterase in the thyroid of the guinea-pig. A histochemical study.豚鼠甲状腺中醋酸α-萘酯酶的巯基。一项组织化学研究。
Histochemistry. 1976 Oct 22;49(2):145-55. doi: 10.1007/BF00495678.
本文引用的文献
1
2
Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase.甲磺酸酯作为乙酰胆碱酯酶的不可逆抑制剂
J Biol Chem. 1962 Oct;237:3245-9.
3
An abnormal pattern of product inhibition observed in phosphate acetyltransferase catalysis.在磷酸乙酰转移酶催化过程中观察到的产物抑制异常模式。
Biochem J. 1972 Mar;127(1):319-20. doi: 10.1042/bj1270319.
4
Kinetic studies with phosphotransacetylase. IV. Inhibition by products.
Biochim Biophys Acta. 1972 Aug 28;276(2):383-91. doi: 10.1016/0005-2744(72)90998-9.
5
Structural specificity of coenzyme A for phosphotransacetylase.
Biochim Biophys Acta. 1970 Nov 24;222(2):307-19. doi: 10.1016/0304-4165(70)90118-2.
6
Phosphotransacetylase of Escherichia coli B, purification and properties.
Biochim Biophys Acta. 1969;191(3):550-8. doi: 10.1016/0005-2744(69)90348-9.
7
Kinetic studies with phosphotransacetylase. V. The mechanism of activation by univalent cations.磷酸转乙酰酶的动力学研究。V. 单价阳离子的激活机制。
Biochim Biophys Acta. 1973 Sep 15;321(1):126-42. doi: 10.1016/0005-2744(73)90066-1.
8
Alkylation studies on a reactive histidine in pig heart malate dehydrogenase.猪心苹果酸脱氢酶中活性组氨酸的烷基化研究。
Eur J Biochem. 1970 Sep;15(3):568-73. doi: 10.1111/j.1432-1033.1970.tb01042.x.
Zotero 插件