钙离子与嗜铬颗粒表面的结合。
Calcium-ion binding to the chromaffin-granule surface.
作者信息
Dean P M, Matthews E K
出版信息
Biochem J. 1974 Sep;142(3):637-40. doi: 10.1042/bj1420637.
Abstract
- Ca(2+) ions decreased the surface charge of isolated adrenal-medullary chromaffin granules whether the granules were untreated or previously incubated with neuraminidase. 2. Ca(2+) binding in both cases followed a Langmuir adsorption isotherm. 3. The chromaffin-granule surface was essentially polyanionic with about 10800 anionic sites per granule, of which 4200 sites are capable of binding Ca(2+) ions with an electrochemical free energy of adsorption of -1.32kJ.mol(-1). 4. The surface region of the chromaffin granule was calculated to bind 1306 Ca(2+) ions at 2.2mm-Ca(2+) (ionic strength 0.16mol.litre(-1)). 5. The importance of Ca(2+) binding to the chromaffin-granule surface is discussed in relation to the hypothesis of secretion by exocytosis.
摘要
- 无论分离出的肾上腺髓质嗜铬颗粒是未经处理的还是先前用神经氨酸酶孵育过的，钙离子都会降低其表面电荷。2. 在这两种情况下，钙离子的结合均遵循朗缪尔吸附等温线。3. 嗜铬颗粒表面本质上是多阴离子的，每个颗粒约有10800个阴离子位点，其中4200个位点能够结合钙离子，吸附的电化学自由能为-1.32kJ·mol⁻¹。4. 计算得出，在2.2mmol/L钙离子（离子强度0.16mol/L）条件下，嗜铬颗粒表面区域可结合1306个钙离子。5. 结合胞吐分泌假说，讨论了钙离子与嗜铬颗粒表面结合的重要性。
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The surface of the washed human erythrocyte as a polyanion.经洗涤的人红细胞表面作为一种聚阴离子。
J Gen Physiol. 1960 Nov;44(2):251-68. doi: 10.1085/jgp.44.2.251.
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Calcium binding by subcellular fractions of bovine adrenal medulla.
J Cell Physiol. 1967 Jun;69(3):311-9. doi: 10.1002/jcp.1040690307.
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A new and simple method for isolation of adrenal chromaffin granules by means of an isotonic density gradient.一种通过等渗密度梯度分离肾上腺嗜铬颗粒的新的简单方法。
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