Conway de Macario E, Heumann A M, Macario A J, Biozzi G
Eur J Immunol. 1979 Mar;9(3):231-6. doi: 10.1002/eji.1830090312.
Amount and affinity of anti-beta-D-galactosidase-activating antibodies (AAb) were studied in two lines of mice obtained by selective breeding for antibody response to heterologous erythrocytes. These lines, referred to as high (H) and low (L) lines, differ also in their responses to many antigens. Concerning AAb amounts, the maximal titer in H mice is higher than in L mice. However, the AAb levels in H mice decreased with increasing immunizing doses and became lower than in L mice for the highest dose. This fact can be explained in terms of intramolecular competition or suppression. Whereas the usual maturation pattern was observed in H mice, no AAb of high affinity were found in L mice, even after a minimal immunizing dose. At any dose tested, no maturation of AAb occurred until three months after immunization. This deficiency may be ascribed to B lymphocyte or macrophage modifications. A correlation between the deficiency of high-affinity AAb in L mice and their genetically induced low antibody responsiveness cannot be proved.
通过对异源红细胞抗体反应进行选择性育种获得的两个品系小鼠中，研究了抗β-D-半乳糖苷酶激活抗体（AAb）的量和亲和力。这两个品系，分别称为高反应（H）品系和低反应（L）品系，它们对许多抗原的反应也有所不同。关于AAb的量，H品系小鼠的最大滴度高于L品系小鼠。然而，H品系小鼠的AAb水平随着免疫剂量的增加而降低，对于最高剂量，其AAb水平低于L品系小鼠。这一事实可以用分子内竞争或抑制来解释。虽然在H品系小鼠中观察到了通常的成熟模式，但即使给予最小免疫剂量后，L品系小鼠中也未发现高亲和力的AAb。在任何测试剂量下，直到免疫后三个月才出现AAb的成熟。这种缺陷可能归因于B淋巴细胞或巨噬细胞的改变。无法证明L品系小鼠中高亲和力AAb的缺乏与其遗传诱导的低抗体反应性之间存在相关性。
