The morphology of cytoplasts and karyoplasts produced by cytochalasin D.
作者信息
Skinnider L
出版信息
Experientia. 1979 Mar 15;35(3):426-7. doi: 10.1007/BF01964390.
PMID:446642
Abstract
The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).
摘要
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