Jenks B G, Meulepas W J, Soons P J, van Overbeeke A P
Mol Cell Endocrinol. 1979 Feb;13(2):149-58. doi: 10.1016/0303-7207(79)90015-7.
Protein biosynthesis in neurointermediate lobes of mouse pituitaries was investigated using pulse and pulse-chase techniques with [3H]lysine. Electrophoretic analysis of lobe homogenates on acid-urea gels resolved 11 labeled products. One was a large protein which was rapidly synthesized during pulse-incubations and disappeared during chase incubations. Three of the products increased during chase incubations, suggesting a precursor-product mode of biosynthesis for these chasde peptides. One of these three products co-migrated with synthetic alpha-MSH and also corresponds to the major peak of mouse neurointermediate lobe MSH bioactivity and immunoactivity on electrophoretograms. Another case of these peptides has electrophoretic properties similar to those of ACTH.
利用[3H]赖氨酸的脉冲和脉冲追踪技术，对小鼠垂体神经中间叶中的蛋白质生物合成进行了研究。在酸性尿素凝胶上对叶匀浆进行电泳分析，分离出11种标记产物。其中一种是大蛋白，在脉冲孵育期间迅速合成，在追踪孵育期间消失。三种产物在追踪孵育期间增加，表明这些追踪肽的生物合成存在前体-产物模式。这三种产物中的一种与合成的α-MSH共迁移，并且在电泳图上也对应于小鼠神经中间叶MSH生物活性和免疫活性的主峰。这些肽的另一个例子具有与促肾上腺皮质激素相似的电泳性质。
