Determination of sulthiame, tetrahydro-2-p-sulphamoyl-phenyl-2h-1,2-thiazine-1,1-dioxide, in plasma at therapeutic concentrations, using high-performance liquid chromatography.

A specific and sensitive liquid chromatographic method for the determination of sulthiame in small (0.5 ml) plasma samples is described. After adding an internal standard, a direct extract of the sample is examined by reversed-phase liquid chromatography with ultraviolet spectrophotometric detection. The method is rapid, simple and capable of determining plasma levels after therapeutic ingestion of sulthiame.