Davidson M B, Casanello-Ertl D
Horm Metab Res. 1979 Mar;11(3):207-9. doi: 10.1055/s-0028-1092708.
Rat myoblasts grown in culture for 5 days responded acutely to insulin (10 mU/ml) over a 3 hour incubation period by stimulating C14-glucose incorporation into glycogen by 260%. When these cells were grown in the presence of insulin (10 mU/ml) for the first 4 days (no exposure to insulin during the final 24 hours), insulin had no significant acute effect. These data provide direct evidence that insulation itself may induce antagonism under certain conditions.
在培养皿中生长5天的大鼠成肌细胞，在3小时的孵育期内对胰岛素（10 mU/ml）产生急性反应，使14C-葡萄糖掺入糖原的量增加了260%。当这些细胞在最初4天处于胰岛素（10 mU/ml）存在的环境中生长（最后24小时不接触胰岛素）时，胰岛素没有显著的急性作用。这些数据提供了直接证据，表明在某些条件下胰岛素本身可能会诱导拮抗作用。
