Piperno G, Bertazzoni U, Berns A J, Bloemendal H
Nucleic Acids Res. 1974 Feb;1(2):245-55. doi: 10.1093/nar/1.2.245.
Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for the excess nucleotides in this message.
小牛晶状体信使核糖核酸级分已在聚(dT)-纤维素上进行了纯化。纯化后的核糖核酸已通过一种可用于微量分析且结果高度可重复的方法被分析为核苷。10S和14S晶状体信使核糖核酸均含有多聚腺苷酸序列。在14S信使核糖核酸中发现的多聚腺苷酸含量无法解释该信使中多余的核苷酸。