Houghton M, Cox R F
Nucleic Acids Res. 1974 Feb;1(2):299-308. doi: 10.1093/nar/1.2.299.
Hen oviduct form B DNA-dependent RNA polymerase has been extensively purified and its properties analysed. It seems likely to consist of a mixture of two forms of the type observed in tissues from other species. Furthermore using S1 nuclease to digest single-stranded DNA, we show that although form B can transcribe double-stranded DNA template it has a very strong preference for single-stranded regions. Also the rate of elongation on native DNA in vitro was measured and is an order of magnitude slower than that reported to be operative in vivo.
母鸡输卵管B型DNA依赖性RNA聚合酶已被广泛纯化并分析了其性质。它似乎可能由在其他物种组织中观察到的两种类型的混合物组成。此外，使用S1核酸酶消化单链DNA，我们发现尽管B型可以转录双链DNA模板，但它对单链区域有非常强烈的偏好。同时还测量了体外天然DNA上的延伸速率，其比据报道在体内起作用的速率慢一个数量级。
