L-丙氨酸——体外淋巴细胞生长的必需氨基酸。

L-alanine--an essential amino acid for growth of lymphocytes in vitro.

作者信息

Nordlind K, Ernström U, Mutt V

出版信息

Int Arch Allergy Appl Immunol. 1979;59(2):215-21. doi: 10.1159/000232261.

Abstract

A factor an ultrafiltrated (UM 10) extracts of calf thymus, liver and spleen stimulated the uptake of 3H-thymidine in DNA of cultured lymphocytes. The factor was purified by ion-exchange chromatography and gel chromatography and identified as alanine, which is lacking in the culture medium, RPMI 1640. An optimal amount of L-alanine increased the uptake of 3H-thymidine in DNA of lymphocytes from different species by about 100%. L-alanine should be regarded as a growth factor for lymphocytes in vitro and should be added to RPMI 1640, when this tissue culture medium is used for lymphocytes.

摘要

一种从小牛胸腺、肝脏和脾脏超滤（UM 10）提取物中获得的因子可刺激培养淋巴细胞DNA中3H-胸腺嘧啶核苷的摄取。该因子通过离子交换色谱和凝胶色谱进行纯化，并鉴定为丙氨酸，而在培养基RPMI 1640中缺乏这种物质。适量的L-丙氨酸可使不同物种淋巴细胞DNA中3H-胸腺嘧啶核苷的摄取增加约100%。L-丙氨酸应被视为体外淋巴细胞的生长因子，当使用这种组织培养基培养淋巴细胞时，应将其添加到RPMI 1640中。