在分光光度测定法中两种二硫化物对胆碱酯酶活性的影响。
The effects of two disulphides on cholinesterase activity in the spectrophotometric assay.
作者信息
Augustinsson K B, Eriksson H
出版信息
Biochem J. 1974 Apr;139(1):123-7. doi: 10.1042/bj1390123.
Abstract
- 5,5'-Dithiobis-(2-nitrobenzoate) did not influence serum cholinesterase activity, whereas 2,2'-dithiodipyridine had an inhibitory effect. 2. The lowering of the molar extinction coefficients observed in the presence of physostigmine may be a result of a reaction between thiolate ions with carbamate moieties. 3. The use of 5,5'-dithiobis-(2-nitrobenzoate) is still recommended in investigations, especially where the quantitative aspects are significant.
摘要
- 5,5'-二硫代双(2-硝基苯甲酸)不影响血清胆碱酯酶活性，而2,2'-二硫代二吡啶具有抑制作用。2. 在毒扁豆碱存在下观察到的摩尔消光系数降低可能是硫醇盐离子与氨基甲酸酯部分之间反应的结果。3. 在研究中，尤其是在定量方面很重要的情况下，仍然建议使用5,5'-二硫代双(2-硝基苯甲酸)。
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本文引用的文献
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Methods Biochem Anal. 1957;5:1-63. doi: 10.1002/9780470110218.ch1.
3
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Arch Biochem Biophys. 1967 Mar;119(1):41-9. doi: 10.1016/0003-9861(67)90426-2.
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Aromatic thiol esters as substrates in quantitative esterase determinations.芳香硫醇酯作为定量酯酶测定中的底物。
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