蜡样芽孢杆菌外毒素的纯化研究（作者译）
[Studies of the purification of the exotoxin of Bacillus cereus (author's transl)].
作者信息
Katsaras K, Hartwigk H
出版信息
Zentralbl Bakteriol Orig A. 1979;245(3):332-44.
The exotoxins of Bacillus cereus show haemolytic, lethal, Phospholipase-C- and enterotoxigenic activities. The enterotoxigenic activity is regarded to be the factor which causes food poisoning in man. Efforts to purify the B. cereus exotoxins by precipitation with ammonium sulphate and subsequent chromatography on Sephadex-G-75 and Biogel-P-60 columns were partially successfull. Haemolysin and Phospholipase-C could be separated by gel-chromatography, they demonstrated partial identity on gel-diffusion agar. The lethal and enterotoxigenic activities could not be separated from the other toxins and remained heterogenous. No immunological relationship was found between Clostridium Type A alpha toxin and enterotoxin and the exotoxins of B. cereus.
蜡样芽孢杆菌的外毒素具有溶血、致死、磷脂酶 C 和产肠毒素活性。产肠毒素活性被认为是导致人类食物中毒的因素。通过硫酸铵沉淀以及随后在 Sephadex-G-75 和 Biogel-P-60 柱上进行色谱分析来纯化蜡样芽孢杆菌外毒素的努力取得了部分成功。溶血素和磷脂酶 C 可通过凝胶色谱分离，它们在凝胶扩散琼脂上显示出部分同一性。致死和产肠毒素活性无法与其他毒素分离，仍然是异质性的。未发现 A 型产气荚膜梭菌α毒素和肠毒素与蜡样芽孢杆菌外毒素之间存在免疫关系。
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