从兔睾丸中分离精子膜唾液酸糖蛋白自身抗原。
Isolation of a sperm membrane sialoglycoprotein autoantigen from rabbit testes.
作者信息
O'Rand M G, Porter J P
出版信息
J Immunol. 1979 Apr;122(4):1248-54.
A single rabbit sperm membrane autoantigen has been isolated from rabbit testes by immunoadsorbent chromatography, agarose chromatography, and electrophoresis. This rabbit sperm autoantigen (RSA-1) is located in the sperm plasma membrane and blocks the sperm cytotoxic (immobilizing) activity of autoantisera. RSA-1 is a sialoglycoprotein, approximately 40% carbohydrate and containing 0.2 micrograms sialic acid/microgram protein. Its m.w. is estimated at 13,000 +/- 1200 by SDA-PAGE. RSA-1 can self-aggregate into higher m.w. forms. Approximately 0.6 mg of RSA-1 can be isolated from 100 rabbits testes. The isolated RSA-1 shows a 300-fold increase in specific cytotoxic (immobilizing) inhibitory activity over the starting testis pellet material.
通过免疫吸附色谱法、琼脂糖色谱法和电泳法，从兔睾丸中分离出了一种单一的兔精子膜自身抗原。这种兔精子自身抗原（RSA - 1）位于精子质膜中，并能阻断自身抗血清的精子细胞毒性（固定）活性。RSA - 1是一种唾液糖蛋白，约含40%的碳水化合物，每微克蛋白质含有0.2微克唾液酸。通过SDA - PAGE法估计其分子量为13,000±1200。RSA - 1可以自我聚合成更高分子量的形式。从100个兔睾丸中大约可以分离出0.6毫克的RSA - 1。与起始的睾丸沉淀物质相比，分离出的RSA - 1的特异性细胞毒性（固定）抑制活性提高了300倍。
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