Bentley J P
J Invest Dermatol. 1979 Jul;73(1):80-3. doi: 10.1111/1523-1747.ep12532769.
Current knowledge of the structure and the mechanism of formation of the covalent cross-links that fuse individual collagen molecules into a stable fiber is reviewed. Some of the mechanical properties of dermal connective tissue and the way in which these change with age can be correlated with the types of cross-link present in the tissue. Cross-links are routinely detected by treatment of a tissue sample with tritium-labeled borohydride and subsequent isolation and quantification of the cross-linked compound, which has been rendered radioactive by reaction with this reducing compound. After maturity, the number of detectable cross-links decreases even though the mechanical stability of the tissue increases. This anomaly is examined in the light of recent data suggesting that cross-links may be oxidized in vivo and thus become undetectable since they can no longer react with borohydride.
本文综述了将单个胶原分子融合成稳定纤维的共价交联结构及形成机制的现有知识。真皮结缔组织的一些力学性能以及这些性能随年龄变化的方式，可与组织中存在的交联类型相关联。交联通常通过用氚标记的硼氢化物处理组织样本，随后分离和定量经与这种还原化合物反应而具有放射性的交联化合物来检测。成熟后，尽管组织的机械稳定性增加，但可检测到的交联数量却减少。鉴于最近的数据表明交联可能在体内被氧化，因而由于它们不再能与硼氢化物反应而变得无法检测，对这一异常现象进行了研究。
