Buogo A, Eboli V
Appl Microbiol. 1972 Jun;23(6):1149-52. doi: 10.1128/am.23.6.1149-1152.1972.
A method for detecting and measuring air contamination by drugs is described which uses an electrostatic bacterial air sampler, sprayers for micronizing drugs, and Mueller-Hinton medium seeded with a highly susceptible strain of Sarcina lutea. Three antibiotics (penicillin, tetracycline, aminosidine) and a sulfonamide (sulfapyrazine) were identified by pretreating portions of medium, showing no bacterial growth, with penicillinase or p-aminobenzoic acid solution and subsequently determining how both drug(-) susceptible and drug-resistant strains of Staphylococcus aureus were affected by this pretreatment. Quantitative determinations were also attempted by measuring the size of the inhibition zones.
描述了一种检测和测量药物空气污染的方法，该方法使用静电细菌空气采样器、用于将药物微粉化的喷雾器以及接种了高敏感性藤黄八叠球菌菌株的穆勒-欣顿培养基。通过用青霉素酶或对氨基苯甲酸溶液预处理部分未显示细菌生长的培养基，随后确定金黄色葡萄球菌的药物敏感菌株和耐药菌株如何受到这种预处理的影响，从而鉴定出三种抗生素（青霉素、四环素、氨基糖苷）和一种磺胺类药物（磺胺吡嗪）。还尝试通过测量抑菌圈大小进行定量测定。
