正常、短肢型及经5-溴脱氧尿苷处理的软骨细胞在细胞培养中合成硫酸软骨素蛋白聚糖。
Synthesis of proteochondroitin sulfate by normal, nanomelic, and 5-bromodeoxyuridine-treated chondrocytes in cell culture.
作者信息
Palmoski M J, Goetinck P F
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3385-8. doi: 10.1073/pnas.69.11.3385.
Abstract
Chondrocytes grown in cell culture from micromelic chick sterna and 5-bromodeoxyuridine-treated normal cells showed reduced incorporation of sulfate into hyaluronidase-sensitive material as compared to normal chondrocytes. Proteochondroitin sulfate synthesized by normal embryonic chick chondrocytes in cell culture was heterogeneous on chromatography. The major chromatographic component was specifically inhibited in normal chondrocytes treated with 5-bromodeoxyuridine, and this component was absent in nanomelic chondrocytes.
摘要
与正常软骨细胞相比，从短肢鸡胸骨中培养的软骨细胞以及经5-溴脱氧尿苷处理的正常细胞，其硫酸盐掺入对透明质酸酶敏感物质的量减少。在细胞培养中，正常胚胎鸡软骨细胞合成的蛋白聚糖硫酸盐在色谱分析中具有异质性。在用5-溴脱氧尿苷处理的正常软骨细胞中，主要的色谱成分受到特异性抑制，而在短肢软骨细胞中不存在该成分。
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