Ono T, Inoue H, Okada Y
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3680-3. doi: 10.1073/pnas.69.12.3680.
In vitro assembly of tobacco mosaic virus (TMV) from its constituent RNA and protein occurs in two steps: formation of the initial complex by interaction of the 5'-end of TMV-RNA with 20S protein aggregate, and growth of the helical rod by sequential addition of protein subunits.Such a process was also observed in the assembly of cucumber green mottle mosaic virus, which is also a rodshaped virus. The 13S virus-protein aggregate, probably a single-ring structure, is needed for formation of the initial complex, instead of the 20S double-ring structure for TMV. The 13S aggregate cannot effect growth of the initial complex to a long infective rod. Rod elongation takes place by the sequential addition of protein subunits. In general, the assembly of rod-shaped viruses occurs in two steps, and the protein aggregate is essential only for initiation of the reaction, not for rod elongation.
烟草花叶病毒（TMV）由其组成的RNA和蛋白质在体外组装分两步进行：TMV-RNA的5'端与20S蛋白质聚集体相互作用形成初始复合物，以及通过蛋白质亚基的顺序添加使螺旋杆生长。在黄瓜绿斑驳花叶病毒的组装过程中也观察到了这样的过程，黄瓜绿斑驳花叶病毒也是一种杆状病毒。形成初始复合物需要13S病毒蛋白质聚集体，可能是单环结构，而不是TMV的20S双环结构。13S聚集体不能使初始复合物生长为长的感染性杆。杆的伸长通过蛋白质亚基的顺序添加发生。一般来说，杆状病毒的组装分两步进行，蛋白质聚集体仅对反应的起始至关重要，而对杆的伸长则不是必需的。
Zotero 插件