苯巴比妥处理的大鼠肠道刷状缘对甘氨酰脯氨酸和游离氨基酸的摄取。

Influx of glycyl-proline and free amino acids across intestinal brush border of phenobarbital-treated rats.

作者信息

Guandalini S, De Marco F, Antoniello S, Migliavacca M, Cerini R, Cacciatore L, Rubino A, De Ritis F

出版信息

Res Commun Chem Pathol Pharmacol. 1979 Jul;25(1):103-10.

Abstract

In a previous study the authors have shown that treatment with phenobarbital in the rat is followed by a generalized increase of amino acid concentration in the plasma. In order to better clarify this phenomenon, the effect of phenobarbital on intestinal protein absorption was now studied by measuring the influxes of Glycyl-L-Proline, L-Phenylalanine, L-Lysine and L-Glutamic acid across the brush border of jejunum and ileum in rats treated with phenobarbital for two or four days. No significant changes of these influxes were observed in the treated animals as compared to the controls, hence suggesting that the effect of phenobarbital on plasma levels of free amino acids is not mediated by an effect on intestinal absorption. The rate of Glycyl-Proline influx as compared to those of amino acid influxes suggests the occurrence of a carrier-mediated transport process for this dipeptide in the rat intestine as previously shown in the rabbit.

摘要

在之前的一项研究中，作者已经表明，给大鼠使用苯巴比妥治疗后，血浆中氨基酸浓度会普遍升高。为了更好地阐明这一现象，现在通过测量甘氨酰 - L - 脯氨酸、L - 苯丙氨酸、L - 赖氨酸和L - 谷氨酸穿过经苯巴比妥处理两天或四天的大鼠空肠和回肠刷状缘的内流情况，研究了苯巴比妥对肠道蛋白质吸收的影响。与对照组相比，在处理过的动物中未观察到这些内流有显著变化，因此表明苯巴比妥对游离氨基酸血浆水平的影响不是由对肠道吸收的作用介导的。与氨基酸内流速率相比，甘氨酰 - 脯氨酸的内流速率表明，如之前在兔子中所显示的那样，大鼠肠道中存在一种载体介导的二肽转运过程。