重氮乙酰枯草杆菌蛋白酶
Diazoacetyl subtilisin.
作者信息
Stefanovsky Y, Westheimer F H
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1132-6. doi: 10.1073/pnas.70.4.1132.
Abstract
Subtilisin reacts at pH 6.8-7.8 with p-nitrophenyl diazoacetate to release p-nitrophenol and form diazoacetyl subtilisin. Although at pH 7.8 this derivative rapidly undergoes hydrolytic cleavage to regenerate active enzyme, the derivative can be trapped by rapidly lowering the pH to 5. Similarly, with (14)C-labeled p-nitrophenyl diazoacetate, the corresponding radiochemically labeled diazoacetyl enzyme can be prepared. Photolysis of this radioactive derivative incorporates radioactivity into the protein, and subsquent hydrolysis gives rise to several radioactive components, which, however, have not yet been identified.
摘要
枯草杆菌蛋白酶在pH 6.8 - 7.8条件下与对硝基苯基重氮乙酸酯反应，释放出对硝基苯酚并形成重氮乙酰基枯草杆菌蛋白酶。尽管在pH 7.8时，这种衍生物会迅速发生水解裂解以再生活性酶，但通过迅速将pH降至5，该衍生物可以被捕获。同样，用（14）C标记的对硝基苯基重氮乙酸酯，可以制备相应的放射化学标记的重氮乙酰基酶。这种放射性衍生物的光解会使放射性掺入蛋白质中，随后的水解会产生几种放射性成分，然而，这些成分尚未得到鉴定。
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本文引用的文献
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ACETYL-CHYMOTRYPSIN.乙酰胰凝乳蛋白酶
Proc Natl Acad Sci U S A. 1955 Apr 15;41(4):190-6. doi: 10.1073/pnas.41.4.190.
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The photolysis of diazoacetylchymotrypsin.重氮乙酰胰凝乳蛋白酶的光解作用。
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The reaction of p-nitrophenyl esters with chymotrypsin and insulin.对硝基苯酯与胰凝乳蛋白酶和胰岛素的反应。
Biochem J. 1954 Feb;56(2):288-97. doi: 10.1042/bj0560288.
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The reactivity of thiol-subtilisin, an enzyme containing a synthetic functional group.硫醇枯草杆菌蛋白酶（一种含有合成官能团的酶）的反应活性。
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6
The determination of the concentration of hydrolytic enzyme solutions: alpha-chymotrypsin, trypsin, papain, elastase, subtilisin, and acetylcholinesterase.水解酶溶液浓度的测定：α-胰凝乳蛋白酶、胰蛋白酶、木瓜蛋白酶、弹性蛋白酶、枯草杆菌蛋白酶和乙酰胆碱酯酶。
J Am Chem Soc. 1966 Dec 20;88(24):5890-913. doi: 10.1021/ja00976a034.
7
Products from the photolysis of diazoacetyl chymotrypsin.重氮乙酰胰凝乳蛋白酶光解产物。
J Biol Chem. 1966 Jan 25;241(2):421-7.
8
A method for marking the hydrophobic binding sites of enzymes. An insertion into the methyl group of an alanine residue of trypsin.一种标记酶疏水结合位点的方法。对胰蛋白酶丙氨酸残基甲基的插入。
J Am Chem Soc. 1969 Jan 1;91(1):217-8. doi: 10.1021/ja01029a055.
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10
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J Biol Chem. 1971 Jun 25;246(12):3928-33.
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