[鲤鱼（鲤属鲤）抗体异质性的研究。I. 抗二硝基苯抗体的电泳和等电光谱]

[Studies of the antibody heterogeneity in carp (Cyprinus carpio L.). I. Electrophoretic and isoelectric spectra of anti-DNP-antibodies].

作者信息

Machulla H K, Richter R F, Gruhn R, Ambrosius H

出版信息

Acta Biol Med Ger. 1979;38(11-12):1607-14.

PMID:45163

Abstract

Carp IgM as well as carp anti-DNP-antibodies migrate electrophoretically very well as a diffuse band into polyacrylamide gel of large pore size. The isoelectric spectra of the carp anti-DNP-antibodies are heterogeneous and show bands in the pI-range of 4.0 to 6.4. The activity of focused anti-DNP-antibodies could be demonstrated in the pI range between 5.4 to 6.4 even in high antibody dilutions. The investigated structural heterogeneity of the anti-DNP-antibodies of carp is a further proof for the phylogenetically early onset of a large antibody heterogeneity of lower vertebrates.

摘要

鲤鱼免疫球蛋白M以及鲤鱼抗二硝基苯酚抗体在电泳时能很好地以弥散带的形式迁移到孔径较大的聚丙烯酰胺凝胶中。鲤鱼抗二硝基苯酚抗体的等电谱具有异质性，在4.0至6.4的pI范围内呈现条带。聚焦后的抗二硝基苯酚抗体即使在高抗体稀释度下，在5.4至6.4的pI范围内仍可显示活性。所研究的鲤鱼抗二硝基苯酚抗体的结构异质性，进一步证明了低等脊椎动物在系统发育早期就存在大量抗体异质性。

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