两条DNA螺旋的比对：噬菌体λ、186和P2的末端生成酶识别DNA碱基序列的模型
Alignment of two DNA helices: a model for recognition of DNA base sequences by the termini-generating enzymes of phage lambda, 186, and P2.
作者信息
Wang J C, Brezinski D P
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2667-70. doi: 10.1073/pnas.70.9.2667.
Abstract
Based on the 3'- and 5'-terminal sequences of DNA of phage lambda, P2, and 186, a model is proposed for recognition of DNA sequences by enzymes responsible for generation of cohesive ends. Two copies of the cohered ends, either on separate molecules or on a concatemer, are aligned with their helical axes parallel but running in opposite directions. The nicking system is dimeric, with each of the two monomers carrying identical sequence-recognition sites. Two pairs of nicks are introduced into the two aligned DNA molecules by the nicking system. The applicability of this model to other biological processes, such as integration of a viral genome into a host genome and the cutting of concatemeric T7 DNA, is discussed.
摘要
基于噬菌体λ、P2和186的DNA 3'端和5'端序列，提出了一个模型，用于解释负责产生粘性末端的酶对DNA序列的识别机制。无论是在单独的分子上还是在多联体上，两个粘性末端的拷贝以平行但方向相反的螺旋轴排列。切口系统是二聚体，两个单体各自携带相同的序列识别位点。切口系统在两个对齐的DNA分子中引入两对切口。本文还讨论了该模型在其他生物学过程中的适用性，如病毒基因组整合到宿主基因组以及多联体T7 DNA的切割。
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