大鼠生长激素的无细胞合成
Cell-free synthesis of rat growth hormone.
作者信息
Bancroft F C, Wu G J, Zubay G
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3646-9. doi: 10.1073/pnas.70.12.3646.
Abstract
Growth hormone has been synthesized in a cell-free system derived from Krebs II ascites cells, under the direction of RNA prepared from rat pituitary tumor (GC) cells. Growth hormone synthesized in the cell-free system was identified by precipitation with antiserum against growth hormone developed in baboon, followed by electrophoretic analysis of the dissolved precipitate on sodium dodecyl sulfate-polyacrylamide gels. RNA from both the membrane and the post-membrane fractions of the cytoplasm of GC cells stimulated protein synthesis in the cell-free system, but only RNA from the membrane fraction was found to direct the synthesis of growth hormone.
摘要
生长激素是在源自克雷布斯II腹水细胞的无细胞系统中，在由大鼠垂体肿瘤（GC）细胞制备的RNA的指导下合成的。通过用针对狒狒体内产生的生长激素的抗血清沉淀，然后对溶解的沉淀物在十二烷基硫酸钠-聚丙烯酰胺凝胶上进行电泳分析，鉴定了在无细胞系统中合成的生长激素。GC细胞细胞质的膜部分和膜后部分的RNA均刺激了无细胞系统中的蛋白质合成，但仅发现膜部分的RNA指导生长激素的合成。
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