Martial J A, Baxter J D, Goodman H M, Seeburg P H
Proc Natl Acad Sci U S A. 1977 May;74(5):1816-20. doi: 10.1073/pnas.74.5.1816.
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine (10 nM) and dexamethasone (1 micron) stimulated increases in growth hormone production by 2.5- and 3.8-fold, respectively. There were corresponding increases in the capacity of RNA from hormone-treated cells to direct synthesis of pregrowth hormone in a wheat germ cell-free translation system, suggesting hormone-regulated increases in growth hormone mRNA. Hormone-induced changes in mRNA were also demonstrated by determining the kinetics of hybridization of a cDNA probe prepared from RNA enriched (about 70%) for growth hormone translational activity with RNA from control and hormone-treated cells. These results suggest that thyroid and glucocorticoid hormones can regulate growth hormone production by influencing the levels of its mRNA.
甲状腺激素和糖皮质激素可刺激培养的大鼠垂体细胞（GC）合成生长激素。我们比较了这些细胞中生长激素分泌量和信使核糖核酸（mRNA）的变化。三碘甲状腺原氨酸（10纳摩尔）和地塞米松（1微摩尔）分别使生长激素分泌量增加了2.5倍和3.8倍。在无细胞麦胚翻译系统中，经激素处理的细胞的核糖核酸（RNA）指导前生长激素合成的能力也相应增加，这表明激素可调节生长激素信使核糖核酸水平的增加。通过测定从富含（约70%）生长激素翻译活性的核糖核酸制备的互补脱氧核糖核酸（cDNA）探针与对照细胞和经激素处理细胞的核糖核酸杂交的动力学，也证实了激素诱导的信使核糖核酸变化。这些结果表明，甲状腺激素和糖皮质激素可通过影响生长激素信使核糖核酸水平来调节生长激素的分泌。
