Seamans K W, Harms P G, Atkins D T, Fleeger J L
Steroids. 1979 Jan;33(1):55-63. doi: 10.1016/s0039-128x(79)80006-9.
Blood samples were collected from eight mares for ten days before and two days following parturition. Progesterone, 5 alpha-dihydroprogesterone and hydroxy-5 alpha-pregnanones were extracted from serum, separated by Sephadex LH-20 column chromatography and quantitated by radioimmunoassay. Progesterone levels ranged from 8.5 to 4.1 ng/ml among mares with normal parturition and decreased to 1.2 ng/ml after parturition. One mare with a retained placenta maintained the prepartal levels of progesterone into the postpartum period. 5 alpha-dihydroprogesterone increased to a level of 133 ng/ml at 80 hrs before parturition and began decreasing and reached a level of 9.2 ng/ml in the postpartum period. Levels of hydroxy-5 alpha-pregnanones were variable with a high of 1690 ng/ml and declined to a postpartum level of 60 ng/ml. The timing of the decline varied among the mares. The possible source and significance of these progestins remain speculative.
在分娩前10天和分娩后2天从8匹母马采集血样。从血清中提取孕酮、5α-双氢孕酮和羟基-5α-孕烷酮，通过葡聚糖凝胶LH-20柱色谱法分离，并用放射免疫分析法进行定量。正常分娩的母马孕酮水平在8.5至4.1纳克/毫升之间，分娩后降至1.2纳克/毫升。一匹胎盘滞留的母马在产后仍维持产前孕酮水平。5α-双氢孕酮在分娩前80小时升至133纳克/毫升，随后开始下降，产后降至9.2纳克/毫升。羟基-5α-孕烷酮水平变化不定，最高达1690纳克/毫升，产后降至60纳克/毫升。母马之间下降的时间各不相同。这些孕激素的可能来源和意义仍属推测。
