Mullins J T, Ellis E A
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1347-50. doi: 10.1073/pnas.71.4.1347.
Male strains of the water mold Achlya ambisexualis produce antheridial hyphae in response to the steroid hormone antheridiol. The antheridial hypha is postulated to be initiated through a localized wall softening with the enzyme cellulase. Freeze-etch studies of hormone-treated hyphae were conducted to determine if aggregates of vesicles are induced at the location of new antheridial hyphae. Localized aggregates of vesicles were found in conjunction with areas of wall thinning. These data suggest that the processes of vesiculation and secretion provide a mechanism for concentration of cellulase at the site of initiation of antheridial hyphae.
雌雄同体水霉的雄性菌株会响应甾体激素雄器醇而产生雄器菌丝。据推测,雄器菌丝是通过纤维素酶使局部细胞壁软化而起始的。对经激素处理的菌丝进行冷冻蚀刻研究,以确定在新的雄器菌丝位置是否会诱导形成囊泡聚集体。在细胞壁变薄的区域发现了囊泡的局部聚集体。这些数据表明,囊泡形成和分泌过程为纤维素酶在雄器菌丝起始位点的聚集提供了一种机制。