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[人胃黏膜中芳基硫酸酯酶A和B的色谱测定]

[Chromatographic determination of arylsulfatases A and B in human gastric mucosa].

作者信息

Antonucci A, Cavallini M

出版信息

Boll Soc Ital Biol Sper. 1979 May 15;55(9):844-8.

PMID:45247
Abstract

In continuation of a previous work, we have confirmed the occurrence of arylsulfatase A in 4 samples of human gastric mucosa analysed by the chromatographic procedure described by Stevens et all. By using the chromatographic method we have also evidentiated the occurrence of arylsulfatase B, which was not detected by using the method of Baum et all. The B form was lower than the A form in 3 samples while it was higher in another sample. In the latter sample of gastric mucosa it was also detected the unusual form Bm of arylsulfatase. It was concluded that both forms A and B of arylsulfatase are present in human gastric mucosa, in variable amounts and that the simple procedure developed by Baum et all., although suitable for the analysis of these enzymes in the urine, is not useful for the determination of arylsulfate B in the gastric mucosa.

摘要

在前一项工作的延续中,我们通过史蒂文斯等人描述的色谱法,在分析的4份人胃黏膜样本中证实了芳基硫酸酯酶A的存在。通过使用色谱法,我们还证实了芳基硫酸酯酶B的存在,而使用鲍姆等人的方法未检测到该酶。在3份样本中,B型低于A型,而在另一份样本中则较高。在该胃黏膜样本中还检测到了芳基硫酸酯酶的异常Bm型。得出的结论是,芳基硫酸酯酶的A和B两种形式都存在于人胃黏膜中,含量各不相同,并且鲍姆等人开发的简单方法虽然适用于尿液中这些酶的分析,但对于胃黏膜中芳基硫酸酯酶B的测定并无用处。

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