Desiatnichenko K S
Vopr Med Khim. 1979 May-Jun;25(3):255-60.
Insoluble complex protein-lipid-calcium phosphate was formed on neutralization of dental enamel solution. Nonpolar lipids were extracted by chloroform-methanol mixture; the phospholipids maintained their connection with the solid phase. After incubation of the enamel precipitate with an anionic detergent the enamel protein became solubilized and was studied by means of electrophoresis. The role of proteins and lipids in calcification of the dental enamel is discussed. Biological calcification appears to operate via a universal mechanism, as evidenced by similarity in molecular structures of organic mixtures of various mineralized tissues.
在牙釉质溶液中和时形成了不溶性复合蛋白质 - 脂质 - 磷酸钙。用氯仿 - 甲醇混合物提取非极性脂质；磷脂与固相保持连接。在用阴离子洗涤剂孵育牙釉质沉淀物后，牙釉质蛋白溶解并通过电泳进行研究。讨论了蛋白质和脂质在牙釉质钙化中的作用。生物钙化似乎通过一种普遍机制起作用，各种矿化组织的有机混合物的分子结构相似性证明了这一点。
