维生素E缺乏：肝脏黄嘌呤氧化酶蓄积增加的免疫化学证据。
Vitamin E deficiency: immunochemical evidence for increased accumulation of liver xanthine oxidase.
作者信息
Catignani G L, Chytil F, Darby W J
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1966-8. doi: 10.1073/pnas.71.5.1966.
Xanthine oxidase (EC 1.2.3.2) activity increases in liver of rabbits deficient in vitamin E. Immunochemical titration of the enzyme demonstrates that the elevation of activity is due to an increased accumulation of the enzyme protein rather than activation of preexisting enzyme molecules. Immunochemically, xanthine oxidase from deficient animals is indistinguishable from the enzyme from control animals. Incorporation of labeled leucine into immunoprecipitable xanthine oxidase is enhanced in vitamin E-deficient animals. This enhancement is interpreted as indicating that the accumulation of xanthine oxidase molecules reflects an accelerated de novo synthesis of the enzyme.
维生素E缺乏的兔子肝脏中黄嘌呤氧化酶（EC 1.2.3.2）的活性会增加。对该酶进行免疫化学滴定表明，活性升高是由于酶蛋白积累增加，而非预先存在的酶分子被激活。从免疫化学角度来看，缺乏维生素E的动物体内的黄嘌呤氧化酶与对照动物体内的酶并无差异。在维生素E缺乏的动物中，标记亮氨酸掺入可免疫沉淀的黄嘌呤氧化酶的过程增强。这种增强被解释为表明黄嘌呤氧化酶分子的积累反映了该酶从头合成的加速。
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