Giacomoni P U, Le Talaer J Y, Le Pecq J B
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3091-5. doi: 10.1073/pnas.71.8.3091.
E. coli DNA-dependent RNA-polymerase binding sites on DNAs of T5, T7, and lambda coliphages have been isolated according to three different methods in order to analyze the binding sites themselves as well as the nearest neighboring regions. It is shown that the binding sites are regions that are rather rich in G+C, are about 14 base pairs long and are located between DNA sequences highly enriched in A+T. The biological implications of this result are discussed.
为了分析大肠杆菌DNA依赖的RNA聚合酶在T5、T7和λ噬菌体DNA上的结合位点本身以及最近的相邻区域,已根据三种不同方法分离出这些结合位点。结果表明,结合位点是富含G+C的区域,长度约为14个碱基对,位于高度富含A+T的DNA序列之间。本文讨论了这一结果的生物学意义。