人类DNA中数量有限的珠蛋白基因。
A limited number of globin genes in human DNA.
作者信息
Gambino R, Kacian D, O'Donnell J, Ramirez F, Marks P A, Bank A
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3966-70. doi: 10.1073/pnas.71.10.3966.
Abstract
The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with beta(+) thalassemia contained a number of globin gene copies similar to that of normal DNA.
摘要
通过将人脾脏的DNA与用（3）H标记的、与人类珠蛋白mRNA互补的DNA杂交，来测定人类细胞中珠蛋白基因的数量。假设互补DNA与细胞DNA的复性速率相似，在细胞DNA与互补DNA的各种比例下互补DNA的杂交程度表明，单倍体人类基因组中珠蛋白基因拷贝数少于10个。对数据的另一种分析，该分析不涉及关于互补DNA与细胞DNA反应相对速率的假设，表明存在少于20个珠蛋白基因拷贝。从一名β（+）地中海贫血患者脾脏中分离的DNA所含珠蛋白基因拷贝数与正常DNA相似。
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2
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3
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J Clin Invest. 1971 Dec;50(12):2755-60. doi: 10.1172/JCI106778.
4
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J Clin Invest. 1971 Nov;50(11):2458-60. doi: 10.1172/JCI106745.
5
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