[作为离子强度和pH值函数的脱氧核糖核酸酶I和II活性分析]

[Analysis of deoxyribonuclease I and II activity as a function of ionic strength and pH].

作者信息

Gallo A, Sorrentino S, Iorio S, Libonati M

出版信息

Boll Soc Ital Biol Sper. 1979 Oct 15;55(19):2006-11.

Abstract

The action of deoxyribonucleases I and II has been studied as a function of ionic strength and pH, in the light of the theory of the ionic control of biochemical reactions (P. Douzou and P. Maurel (1977) Proc. Nat. Acad. Sci. USA, 74, 1013-1015). The pattern of DNA degradation by the two enzymes fits the general principles of the theory. However, the activity of DNAase II, a dimeric, basic protein (pI = 10,2) appears to be scarcely modulated by variables such as ionic strength and pH. This is reminiscent of what was elsewhere observed with the system double stranded RNA-seminal RNAase (also a very basic, dimeric enzyme), and could, therefore, tentatively be correlated with the dimeric and/or the very basic nature of the enzyme protein.

摘要

根据生化反应离子控制理论（P. 杜佐和P. 莫雷尔（1977年），《美国国家科学院院刊》，74卷，第1013 - 1015页），对脱氧核糖核酸酶I和II的作用作为离子强度和pH值的函数进行了研究。两种酶对DNA的降解模式符合该理论的一般原则。然而，作为一种二聚体碱性蛋白（pI = 10.2）的DNA酶II的活性似乎几乎不受离子强度和pH值等变量的调节。这让人想起在双链RNA - 精液核糖核酸酶系统（也是一种非常碱性的二聚体酶）中在其他地方观察到的情况，因此，可以初步将其与酶蛋白的二聚体和/或非常碱性的性质联系起来。