溴脱氧尿苷对溴脱氧尿苷依赖性细胞的可逆“转化”

Reversible "transformation" of bromodeoxyuridine-dependent cells by bromodeoxyuridine.

作者信息

Davidson R L, Horn D

出版信息

Proc Natl Acad Sci U S A. 1974 Sep;71(9):3338-42. doi: 10.1073/pnas.71.9.3338.

DOI:10.1073/pnas.71.9.3338

PMID:4530304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433766/

Abstract

The growth characteristics of a bromodeoxyuridine-dependent cell line that was derived from a non-contact-inhibited Syrian hamster melanoma line were studied. The dependent cells require high concentrations of bromodeoxyuridine not only for optimal growth but also for the maintenance of the non-contact-inhibited state. When grown in the absence of bromodeoxyuridine, the dependent cells become contact-inhibited. The transition to the contact-inhibited state is reversed when bromodeoxyuridine is added back to the medium, if the bromodeoxyuridine is incorporated into dividing cells. The effects of bromodeoxyuridine on growth rate and on contact inhibition are separable.

摘要

对一株源自非接触抑制性叙利亚仓鼠黑色素瘤细胞系的溴脱氧尿苷依赖性细胞系的生长特性进行了研究。依赖性细胞不仅需要高浓度的溴脱氧尿苷以实现最佳生长，还需要其来维持非接触抑制状态。当在无溴脱氧尿苷的情况下生长时，依赖性细胞会变成接触抑制性的。如果将溴脱氧尿苷重新添加到培养基中且其被掺入正在分裂的细胞中，那么向接触抑制状态的转变就会逆转。溴脱氧尿苷对生长速率和接触抑制的影响是可分离的。