溴脱氧尿苷依赖性——哺乳动物细胞中的一种新突变。
Bromodeoxyuridine dependence--a new mutation in mammalian cells.
作者信息
Davidson R L, Bick M D
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):138-42. doi: 10.1073/pnas.70.1.138.
Abstract
When cells of a Syrian hamster melanoma were grown in increasing concentrations of bromodeoxyuridine (BrdU), lines of mutant cells able to grow well at high concentrations of BrdU were isolated. The mutant cells were characterized by BrdU dependence. In the absence of BrdU, the cells grew very poorly. The requirement for BrdU was specific for both the bromine and the deoxy sugar. The mutant cells incorporated BrdU into the DNA, replacing about 50% of the thymine residues with bromouracil. The reason for the BrdU dependence is not known.
摘要
当叙利亚仓鼠黑色素瘤细胞在浓度不断增加的溴脱氧尿苷（BrdU）中培养时，分离出了能够在高浓度BrdU下良好生长的突变细胞系。这些突变细胞的特征是对BrdU有依赖性。在没有BrdU的情况下，细胞生长非常缓慢。对BrdU的需求对溴和脱氧糖来说都是特定的。突变细胞将BrdU掺入DNA中，用溴尿嘧啶取代了约50%的胸腺嘧啶残基。BrdU依赖性的原因尚不清楚。
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