Branson H E, Slater L M, Anderson M G, Pirkle H
Am J Clin Pathol. 1979 Jun;71(6):665-7. doi: 10.1093/ajcp/71.6.665.
Coumarin-anticoagulated plasmas from 31 subjects comprising two study groups were passed through epichlorohydrin triethanolamine cellulose (ECTEOLA) chromatography columns. Group I plasmas, which were run with nothing added, had mean prothrombin times of 21.6 +/- 5.4 sec prior to and 22.4 +/- 5.6 sec following exposure to these columns (r = 0.9449; t = 1.8307, P less than 0.100). The mean prothrombin time for Group II, 18.2 +/- 4.9 sec, lengthened with 5 u/ml heparin to 35.5 +/- 16.2 sec, and returned to 19.2 +/- 5.0 (r = 0.9763) after chromatography. Therefore, it appears that coumarin anticoagulation had no significant influence upon the capacity of ECTEOLA effectively to remove heparin in therapeutic doses. This means that virtually all prothrombin time reagent systems can be employed to monitor concurrent heparin and coumarin anticoagulation. In addition, quality control of the combined technic is simpler, and the technic more sensitive to low levels of fibrinogen and factor V.
来自包含两个研究组的31名受试者的香豆素抗凝血浆通过环氧氯丙烷三乙醇胺纤维素（ECTEOLA）色谱柱。第一组血浆在未添加任何物质的情况下运行，暴露于这些色谱柱之前的平均凝血酶原时间为21.6±5.4秒，之后为22.4±5.6秒（r = 0.9449；t = 1.8307，P＜0.100）。第二组的平均凝血酶原时间为18.2±4.9秒，加入5 U/ml肝素后延长至35.5±16.2秒，色谱分析后恢复至19.2±5.0（r = 0.9763）。因此，似乎香豆素抗凝对ECTEOLA有效去除治疗剂量肝素的能力没有显著影响。这意味着几乎所有凝血酶原时间试剂系统都可用于监测同时进行的肝素和香豆素抗凝。此外，联合技术的质量控制更简单，并且该技术对低水平纤维蛋白原和因子V更敏感。
