磁圆二色性研究XXXV. 细胞色素P - 450与P - 448的比较
Magnetic circular dichroism studies XXXV. A comparison of cytochromes P-450 and P-448.
作者信息
Dawson J H, Dolinger P M, Trudell J R, Barth G, Linder R E, Bunnenberg E, Djerassi C
出版信息
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4594-7. doi: 10.1073/pnas.71.11.4594.
Cytochromes P-450 and P-448 in microsomal suspensions have been shown to be spectrally distinct by magnetic circular dichroism spectroscopy. Furthermore, this technique can be used to measure induction of these two cytochromes by phenobarbital and 3-methyl-cholanthrene. Magnetic circular dichroism spectroscopy is thus at least as useful as difference spectroscopy for the investigation of P-450 and P-448 and more informative because the presence of cytochrome b(5) and hemoglobin can be detected concurrently. We have also shown that the molar magnetic ellipticity for reduced + CO treated cytochrome P-450 of Pseudomonas grown on camphor is of similar value to that of reduced + CO treated microsomal P-450 and P-448.
微粒体悬浮液中的细胞色素P-450和P-448已通过磁圆二色光谱法显示出光谱上的差异。此外，该技术可用于测量苯巴比妥和3-甲基胆蒽对这两种细胞色素的诱导作用。因此，磁圆二色光谱法在研究P-450和P-448方面至少与差示光谱法一样有用，并且信息更丰富，因为可以同时检测细胞色素b(5)和血红蛋白的存在。我们还表明，在樟脑上生长的假单胞菌中，还原+CO处理的细胞色素P-450的摩尔磁椭圆率与还原+CO处理的微粒体P-450和P-448的摩尔磁椭圆率具有相似的值。
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