与纯化的PM2噬菌体相关的核酸内切酶活性。
Endonuclease activity associated with purified PM2 bacteriophages.
作者信息
Laval F
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4965-9. doi: 10.1073/pnas.71.12.4965.
Abstract
An endonucleolytic activity is associated with purified PM(2) bacteriophages. It converts the double-stranded supercoiled PM(2) DNA mostly into the linear form that sediments as a homogenous peak in alkaline sucrose gradients. The same activity is found in intact or detergent-lysed phages. Optimal activity is observed between pH 6.8 and 7.5 at 28 degrees . Divalent cations, Mg(2+) or Mn(2+), are necessary for activity, and the enzyme is inhibited by RNA. The endonuclease has no appreciable activity on linear DNA molecules, but it attacks single-stranded circular DNA.
摘要
核酸内切酶活性与纯化的PM(2)噬菌体相关。它将双链超螺旋PM(2) DNA大部分转化为线性形式，该线性形式在碱性蔗糖梯度中沉降为单一峰。在完整的或经去污剂裂解的噬菌体中也发现了相同的活性。在28℃时，pH 6.8至7.5之间观察到最佳活性。二价阳离子Mg(2+)或Mn(2+)对活性是必需的，并且该酶受到RNA的抑制。这种核酸内切酶对线性DNA分子没有明显活性，但它会攻击单链环状DNA。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
A rapidly labeled RNA in rat liver nuclei.
J Mol Biol. 1962 Aug;5:217-29. doi: 10.1016/s0022-2836(62)80085-0.
3
Properties of bacteriophage PM2: a lipid-containing bacterial virus.
Virology. 1968 Apr;34(4):738-47. doi: 10.1016/0042-6822(68)90094-9.
4
Replication of bacteriophage PM2 deoxyribonucleic acid: a closed circular double-stranded molecule.
J Mol Biol. 1971 Mar 28;56(3):597-621. doi: 10.1016/0022-2836(71)90404-9.
5
Structure and synthesis of a lipid-containing bacteriophage. 3. Purification of bacteriophage PM2 and some structural studies on the virion.
Virology. 1969 Dec;39(4):666-81. doi: 10.1016/0042-6822(69)90005-1.
6
DNA of bacteriophage PM2: a closed circular double-stranded molecule.
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8. doi: 10.1073/pnas.63.4.1164.
7
Structure and synthesis of a lipid-containing bacteriophage. VII. Structural proteins of bacteriophage PM2.
Virology. 1971 Jul;45(1):232-9. doi: 10.1016/0042-6822(71)90130-9.
8
Purification and properties of an acid deoxyribonuclease from the snail Helix aspersa Müll.
Biochemistry. 1972 Sep 12;11(19):3596-603. doi: 10.1021/bi00769a016.
9
Structure and synthesis of a lipid-containing bacteriophage. X. Improved techniques for the purification of bacteriophage PM2.
Virology. 1972 Apr;48(1):259-62. doi: 10.1016/0042-6822(72)90133-x.
10
Use of ethidium bromide for separation and determination of nucleic acids of various conformational forms and measurement of their associated enzymes.
Methods Biochem Anal. 1971;20:41-86. doi: 10.1002/9780470110393.ch2.
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