Byram J E, Senft A W
Am J Trop Med Hyg. 1979 May;28(3):539-47. doi: 10.4269/ajtmh.1979.28.539.
Preparations of eggshells of Schistosoma mansoni and S. japonicum were hydrolyzed and analyzed for amino acid composition. Both species showed great similarities in the proportions of each residue found. The predominant amino acid in shell hydrolysates was found to be glycine, which accounted for 37% of S. mansoni and 45% of S. japonicum amino acids. Four components (glycine, aspartic acid, lysine, and serine) totalled 68--75% of amino acids in the eggshells. Other individual amino acids were present in relatively small proportions ranging from 5.2--0.01%. Less than 1% of the amino acid residues were identified as tyrosine, and bityrosine was detected at a level not exceeding 1 in 1,600 residues. Carbohydrates were estimated to comprise 7.5--10% of the eggshell weight, based on hexose assay, and glucosamine was identified as the principal amino sugar in shell hydrolysates. In vivo labelling of the S. mansoni eggshell was demonstrated following injection of C14-glycine and C14-tyrosine into infected mice and subsequent purification of the shells of eggs recovered from their liver.
对曼氏血吸虫和日本血吸虫的卵壳制剂进行水解，并分析其氨基酸组成。在每种残留成分的比例方面，这两种血吸虫显示出极大的相似性。发现卵壳水解产物中的主要氨基酸是甘氨酸，在曼氏血吸虫氨基酸中占37%，在日本血吸虫氨基酸中占45%。四种成分（甘氨酸、天冬氨酸、赖氨酸和丝氨酸）占卵壳氨基酸总量的68% - 75%。其他单个氨基酸的比例相对较小，范围从5.2%到0.01%。被鉴定为酪氨酸的氨基酸残基不到1%，并且检测到的双酪氨酸水平不超过1600个残基中的1个。基于己糖测定法，估计碳水化合物占卵壳重量的7.5% - 10%，并且氨基葡萄糖被鉴定为卵壳水解产物中的主要氨基糖。在向感染小鼠注射C14 - 甘氨酸和C14 - 酪氨酸并随后从其肝脏中回收的卵的壳进行纯化之后，证明了曼氏血吸虫卵壳的体内标记。
