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本文引用的文献

1
Current-voltage relations in the lobster giant axon membrane under voltage clamp conditions.电压钳制条件下龙虾巨轴突膜的电流-电压关系
J Gen Physiol. 1962 Jul;45(6):1217-38. doi: 10.1085/jgp.45.6.1217.
2
The influence of potassium and chloride ions on the membrane potential of single muscle fibres.钾离子和氯离子对单根肌纤维膜电位的影响。
J Physiol. 1959 Oct;148(1):127-60. doi: 10.1113/jphysiol.1959.sp006278.
3
THE RUBIDIUM AND POTASSIUM PERMEABILITY OF FROG MUSCLE MEMBRANE.青蛙肌肉膜的铷和钾通透性
J Physiol. 1964 Dec;175(1):134-59. doi: 10.1113/jphysiol.1964.sp007508.
4
'Glycerol effect' and the mechanism linking excitation of the plasma membrane with contraction.“甘油效应”以及将质膜兴奋与收缩联系起来的机制。
Nature. 1961 Dec 23;192:1159-61. doi: 10.1038/1921159a0.
5
Potassium conductance of frog muscle membrane under controlled voltage.在可控电压下青蛙肌肉膜的钾离子电导
J Physiol. 1962 Aug;163(1):104-14. doi: 10.1113/jphysiol.1962.sp006960.
6
Analysis of certain errors in squid axon voltage clamp measurements.鱿鱼轴突电压钳测量中某些误差的分析。
Biophys J. 1960 Nov;1(2):161-202. doi: 10.1016/s0006-3495(60)86882-8.
7
Negative after-potential of frog's skeletal muscle.青蛙骨骼肌的负后电位
J Neurophysiol. 1957 Nov;20(6):602-14. doi: 10.1152/jn.1957.20.6.602.
8
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
9
Frog skeletal muscle fibers: changes in electrical properties after disruption of transverse tubular system.青蛙骨骼肌纤维：横管系统破坏后电特性的变化
Science. 1967 Dec 29;158(3809):1700-1. doi: 10.1126/science.158.3809.1700.
10
Membrane currents at large positive internal potentials in single myelinated nerve fibres of Rana pipiens.牛蛙单根有髓神经纤维在大的正性膜内电位时的膜电流。
J Physiol. 1967 Nov;193(2):433-42. doi: 10.1113/jphysiol.1967.sp008368.

牛蛙单根肌纤维的电压钳实验。

Voltage clamp experiments of single muscle fibers of Rana pipiens.

作者信息

Moore L E

出版信息

J Gen Physiol. 1972 Jul;60(1):1-19. doi: 10.1085/jgp.60.1.1.

DOI:10.1085/jgp.60.1.1
PMID:4537778
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2226057/
Abstract

A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These currents looked qualitatively similar when the transverse tubular system was destroyed. Errors in current measurement, especially those due to anomalous rectification, are discussed.

摘要

已开发出一种用于单根肌纤维的电压钳。当通过将单根肌纤维包裹在凡士林密封中创建一个人工节点来实现系统稳定性时，该凡士林密封充当髓鞘的类似物。典型的电压钳记录显示，先是出现大的内向瞬态电流，随后是外向电流的延迟整流。当横管系统被破坏时，这些电流在性质上看起来相似。文中讨论了电流测量中的误差，特别是由反常整流引起的误差。