牛蛙单根肌纤维的电压钳实验。
Voltage clamp experiments of single muscle fibers of Rana pipiens.
作者信息
Moore L E
出版信息
J Gen Physiol. 1972 Jul;60(1):1-19. doi: 10.1085/jgp.60.1.1.
Abstract
A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These currents looked qualitatively similar when the transverse tubular system was destroyed. Errors in current measurement, especially those due to anomalous rectification, are discussed.
摘要
已开发出一种用于单根肌纤维的电压钳。当通过将单根肌纤维包裹在凡士林密封中创建一个人工节点来实现系统稳定性时，该凡士林密封充当髓鞘的类似物。典型的电压钳记录显示，先是出现大的内向瞬态电流，随后是外向电流的延迟整流。当横管系统被破坏时，这些电流在性质上看起来相似。文中讨论了电流测量中的误差，特别是由反常整流引起的误差。
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