Hsieh H C, Stoll N R, Reber E W, Chen E R, Kang B T, Kuo M
Bull World Health Organ. 1972;47(3):317-24.
In the first country-wide survey of hookworm infection in Liberia, diagnosis by test-tube filter-paper cultivation revealed a prevalence of more than 90%. This sensitive technique also permitted differential species determination. Necator americanus was found to occur in all regions and in a high proportion of the persons examined. Ancylostoma duodenale was mainly confined to a narrow coastal belt, and usually parasitized individuals harbouring N. americanus as well. The average number of eggs per gram of faeces (EPG) of infected individuals was lowest in the population of the hinterland. During the latter part of the studies a modified test-tube filter-paper cultivation technique was used in combination with dilution counting to obtain data on intensity of infection separately for the 2 species. For N. americanus there was little regional difference in the intensity of infection, but the EPG for A. duodenale was much higher in people living in the coastal area.
在利比里亚首次开展的全国性钩虫感染调查中，通过试管滤纸培养法进行诊断发现感染率超过90%。这种灵敏的技术还能对不同种类进行鉴别。研究发现，美洲板口线虫在所有地区以及大部分受检人员中都有出现。十二指肠钩口线虫主要集中在狭窄的沿海地带，而且通常寄生于同时感染美洲板口线虫的个体。内陆地区受感染个体每克粪便中的虫卵平均数（EPG）最低。在研究后期，采用了改良的试管滤纸培养技术并结合稀释计数法，以分别获取这两种钩虫感染强度的数据。美洲板口线虫的感染强度在各地区差异不大，但沿海地区居民十二指肠钩口线虫的EPG要高得多。
