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哺乳动物DNA的粘弹性行为。

Viscoelastic behavior of mammalian DNA.

作者信息

Chase E S, Shafer R H

出版信息

Biophys J. 1979 Oct;28(1):93-105. doi: 10.1016/S0006-3495(79)85161-9.

DOI:10.1016/S0006-3495(79)85161-9
PMID:45402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1328612/
Abstract

The viscoelastic behavior of rat 9L cellular DNA was studied as a function of the detergent used for lysis, the pH and duration of lysis, and gamma ray dose. For nondenaturing lysis conditions, a model of the DNA was proposed to account for the effects of these agents on the viscoelastic retardation time. It was concluded that these agents affect the hydrodynamic radius of the DNA rather than its molecular weight. For denaturing lysis conditions, molecular weights calculated from the relaxation time were consistent with those calculated from alkaline sucrose sedimentation profiles.

摘要

研究了大鼠9L细胞DNA的粘弹性行为与用于裂解的去污剂、裂解的pH值和持续时间以及γ射线剂量之间的关系。对于非变性裂解条件，提出了一个DNA模型来解释这些因素对粘弹性弛豫时间的影响。得出的结论是，这些因素影响DNA的流体动力学半径而非其分子量。对于变性裂解条件，根据弛豫时间计算出的分子量与根据碱性蔗糖沉降图谱计算出的分子量一致。

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本文引用的文献

1
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
A marker for mammalian DNA sedimentation.一种用于哺乳动物DNA沉降的标记物。
Radiat Res. 1974 Mar;57(3):365-78.
3
Sedimentation of DNA from human fibroblasts irradiated with ultraviolet light: possible detection of excision breaks in normal and repair-deficient xeroderma pigmentosum cells.紫外线照射后人成纤维细胞中DNA的沉降：正常及色素性干皮病修复缺陷细胞中切除断裂的可能检测
Radiat Res. 1974 Feb;57(2):207-27.
4
Sensitivity of micrurgically removed chromosomal spindle fibres to enzyme disruption.显微手术切除的染色体纺锤体纤维对酶破坏的敏感性。
Nature. 1968 Feb 24;217(5130):748-50. doi: 10.1038/217748a0.
5
Sedimentation of DNA released from Chinese hamster cells.从中国仓鼠细胞中释放的DNA的沉降。
Biophys J. 1971 Jun;11(6):502-20. doi: 10.1016/S0006-3495(71)86231-8.
6
Conformation of DNA in alkaline sucrose: the subunit hypothesis in mammalian cells.碱性蔗糖中DNA的构象：哺乳动物细胞中的亚基假说
Biochem Biophys Res Commun. 1974 Jul 10;59(1):92-9. doi: 10.1016/s0006-291x(74)80179-8.
7
Interphase chromosomal deoxyribonucleoprotein isolated as a discrete structure from cultured cells.从培养细胞中分离出的作为离散结构的间期染色体脱氧核糖核蛋白。
J Mol Biol. 1974 Jul 5;86(3):649-63. doi: 10.1016/0022-2836(74)90187-9.
8
Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli.通过粘弹性测量确定的DNA大小：噬菌体、枯草芽孢杆菌和大肠杆菌的测量结果
J Mol Biol. 1972 Dec 30;72(3):779-800. doi: 10.1016/0022-2836(72)90191-x.
9
Radiation induced strand breakage in DNA from mammalian cells. Strand separation in alkaline solution.辐射诱导哺乳动物细胞DNA链断裂。碱性溶液中的链分离。
Int J Radiat Biol Relat Stud Phys Chem Med. 1973 Mar;23(3):285-9. doi: 10.1080/09553007314550311.
10
The effect of oxygen on DNA single-strand breaks produced by ionizing radiation in mammalian cells.氧气对哺乳动物细胞中电离辐射产生的DNA单链断裂的影响。
Int J Radiat Biol Relat Stud Phys Chem Med. 1972 May;21(5):417-33. doi: 10.1080/09553007214550501.

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