Strober S, Dilley J
J Exp Med. 1973 May 1;137(5):1275-92. doi: 10.1084/jem.137.5.1275.
The adoptive secondary antibody response of rats to the hapten-protein conjugate dinitrophenyl-diphtheria toxoid (DNP-DT) was used to investigate the migratory properties and rate of formation of T and B memory cells in the spleen. The experimental findings show that hapten (DNP-BSA)- and carrier (DT)-primed spleen cells act synergistically in the restoration of the adoptive anti-DNP response. Passage of both hapten- and carrier-primed spleen cells through an intermediate host (intravenous injection and subsequent collection in the thoracic duct lymph) showed that both cell types are able to recirculate from the blood to the lymph. In addition, memory to the hapten or carrier could be withdrawn from the spleen by prolonged thoracic duct drainage. The rate of formation of hapten- and carrier-primed spleen cells was studied by treating donors with [(3)H]thymidine for 48 h before cell transfer in an attempt to "suicide" rapidly dividing cells. Only a slight reduction in the adoptive response to the hapten or carrier was noted upon transfer of treated cells to irradiated hosts. In further experiments, the cell lineage of hapten- and carrier-primed cells was determined by treating each cell type in vitro with rabbit antirat B cell serum (RARBS) and complement. Although treatment with RARBS did not affect the adoptive response restored by carrier-primed cells, the same treatment abolished the response restored by hapten-primed cells. These findings indicate that T and B memory cells in the spleen of the rat are relatively long-lived, recirculating lymphocytes. The contribution of fixed or rapidly turning over cells to immunological memory is small or negligible as compared with the latter cells.
利用大鼠对半抗原 - 蛋白质偶联物二硝基苯基 - 白喉类毒素（DNP - DT）的过继性二次抗体反应，来研究脾脏中T和B记忆细胞的迁移特性及形成速率。实验结果表明，经半抗原（DNP - BSA）和载体（DT）致敏的脾细胞在恢复过继性抗DNP反应中起协同作用。使经半抗原和载体致敏的脾细胞通过中间宿主（静脉注射，随后收集胸导管淋巴液），结果显示这两种细胞类型都能够从血液再循环至淋巴。此外，通过长时间的胸导管引流可使脾脏对半抗原或载体的记忆消除。通过在细胞转移前用[³H]胸腺嘧啶核苷处理供体48小时，以试图“清除”快速分裂的细胞，从而研究经半抗原和载体致敏的脾细胞的形成速率。将处理过的细胞转移至受照射宿主后，对该半抗原或载体的过继性反应仅略有降低。在进一步的实验中，通过在体外用兔抗大鼠B细胞血清（RARBS）和补体处理每种细胞类型，来确定经半抗原和载体致敏的细胞的细胞谱系。虽然用RARBS处理不影响经载体致敏的细胞恢复的过继性反应，但相同处理却消除了经半抗原致敏的细胞恢复的反应。这些发现表明，大鼠脾脏中的T和B记忆细胞是相对长寿的再循环淋巴细胞。与后一种细胞相比，固定细胞或快速更新的细胞对免疫记忆的贡献很小或可忽略不计。
