一种分离肾刷状缘膜的快速方法。
A rapid method for the isolation of kidney brush border membranes.
作者信息
Malathi P, Preiser H, Fairclough P, Mallett P, Crane R K
出版信息
Biochim Biophys Acta. 1979 Jun 13;554(1):259-63. doi: 10.1016/0005-2736(79)90023-3.
PMID:454602
Abstract
A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.
摘要
本文描述了一种从兔肾近端小管制备纯化刷状缘膜的简单快速方法。该方法基于低渗裂解、污染物的Ca2+聚集和差速离心。与大多数其他已发表的方法不同，刷状缘膜不受基底外侧膜的污染。
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