Arányi P
Biochim Biophys Acta. 1979 May 16;584(3):529-37. doi: 10.1016/0304-4165(79)90125-9.
The usual way of in vitro determination of association constants for hormone-receptor complexes is criticized. It is shown that if incubation time is short, relative to the half-life of the hormone-receptor complex, the value of the apparent Ka is proportional to the time of incubation. No sign of lack of equilibrium is apparent from the Scatchard plots. The case of rapidly denaturing receptor molecule is also discussed, with similar conclusions. Although terminology and examples are taken from the field of the glucocorticoid receptor research, all deductions are valid for other systems with similar association (and denaturation or monomolecular transformation) mechanisms and kinetic parameters.
体外测定激素-受体复合物结合常数的常用方法受到了批评。结果表明，如果孵育时间相对于激素-受体复合物的半衰期较短，表观解离常数（Ka）的值与孵育时间成正比。从Scatchard图中没有明显的平衡缺乏迹象。文中还讨论了受体分子快速变性的情况，得出了类似的结论。虽然术语和例子取自糖皮质激素受体研究领域，但所有推论对于具有相似结合（以及变性或单分子转化）机制和动力学参数的其他系统都是有效的。
