用流感嗜血杆菌的限制性内切酶分析真核生物DNA：“隐蔽”卫星DNA的分离。

Analysis of eucaryotic DNAs with a restriction endonuclease from H. influenzae: isolation of "hidden" satellite DNAs.

作者信息

Roizés G

出版信息

Nucleic Acids Res. 1974 Sep;1(9):1099-120. doi: 10.1093/nar/1.9.1099.

DOI:10.1093/nar/1.9.1099

PMID:4548936

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344334/

Abstract

The restriction enzymes from H.influenzae have been used to study various eucaryotic DNAs. Fractions resistant to the action of these enzymes have been isolated from rat, mouse and calf DNAs. The method revealed the existence in rat of several components having the properties of satellite DNAs. Mouse DNA was shown to contain a new satellite of buoyant density 1. 704. Therefore, this appears to be a powerfull method for the isolation of "hidden" satellite DNAs. Calf satellite DNAs rho = 1.705 and rho = 1.723 were those resistant to the action of the restriction enzymes whilst satellite DNAs rho = 1.714 and rho = 1.710 gave fragments of discrete lengths, suggesting internal repeat units of 1 500 and 5 000 base pairs respectively.

摘要

来自流感嗜血杆菌的限制性内切酶已被用于研究各种真核生物的DNA。已从大鼠、小鼠和小牛的DNA中分离出对这些酶的作用具有抗性的组分。该方法揭示了大鼠中存在几种具有卫星DNA特性的成分。小鼠DNA被证明含有一种浮力密度为1.704的新卫星DNA。因此，这似乎是一种分离“隐藏”卫星DNA的有效方法。小牛的卫星DNA ρ=1.705和ρ=1.723对限制性内切酶的作用具有抗性，而卫星DNA ρ=1.714和ρ=1.710产生了离散长度的片段，分别表明内部重复单元为1500和5000个碱基对。