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两种用于检测和计数尿液样本中埃及血吸虫卵的新现场技术,并对两种方法进行评估。

Two new field techniques for detection and counting of Schistosoma haematobium eggs in urine samples, with an evaluation of both methods.

作者信息

Dazo B C, Biles J E

出版信息

Bull World Health Organ. 1974;51(4):399-408.

PMID:4549491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2366293/
Abstract

Two new techniques for the quantitative determination of Schistosoma haematobium egg densities in urine samples, the first using sedimentation and the second filtration, have been developed and evaluated. In the first method the concentrated sediment is examined in a counting chamber under a low-power microscope and in the second method the final concentration achieved by filtration is also examined microscopically. Both procedures are easily carried out under primitive field conditions, while retaining their reproducibility and reliability. The techniques can be useful in mass surveys of populations suffering from schistosomiasis.

摘要

已研发并评估了两种用于定量测定尿样中埃及血吸虫卵密度的新技术,第一种采用沉淀法,第二种采用过滤法。在第一种方法中,将浓缩沉淀物置于低倍显微镜下的计数室中进行检查,在第二种方法中,通过过滤获得的最终浓缩物也通过显微镜进行检查。这两种方法在原始野外条件下都易于实施,同时保持其可重复性和可靠性。这些技术在血吸虫病流行人群的大规模调查中可能会有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed71/2366293/450ab6620d41/bullwho00470-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed71/2366293/517d7af26d68/bullwho00470-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed71/2366293/450ab6620d41/bullwho00470-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed71/2366293/517d7af26d68/bullwho00470-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed71/2366293/450ab6620d41/bullwho00470-0076-a.jpg

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