一种测定阿莫地喹的方法。
A method for the determination of amodiaquine.
作者信息
Trenholme G M, Williams R L, Patterson E C, Frischer H, Carson P E, Rieckmann K H
出版信息
Bull World Health Organ. 1974;51(4):431-4.
A new fluorometric method for analysis of amodiaquine in serum, plasma, or red cells is described. Amodiaquine is extracted from alkalinized biological fluid into 1,2-dichloroethane and is then re-extracted into 0.1 N hydrochloric acid. Borate buffer is added to the acid solution and the resultant solution is heated for 30 min in boiling water. Heating the buffered solution produces a marked increase in the fluorescence of amodiaquine, which may then be measured. Standard curves prepared in serum and red cells were linear between 50 and 3 000 mug/litre. Reproducibility of the assay and recovery of amodiaquine from serum and red cells were satisfactory. The specificity of the assay and the nature of the induced fluorophor are not known. The paper indicates representative serum and red cell levels of amodiaquine after the administration to 5 subjects of 10 mg of amodiaquine base per kg of body weight.
本文描述了一种用于分析血清、血浆或红细胞中阿莫地喹的新型荧光测定法。将阿莫地喹从碱化的生物流体中萃取至1,2 - 二氯乙烷中，然后再萃取至0.1N盐酸中。向酸性溶液中加入硼酸盐缓冲液，所得溶液在沸水中加热30分钟。加热缓冲溶液会使阿莫地喹的荧光显著增强，随后即可进行测量。在血清和红细胞中制备的标准曲线在50至3000微克/升之间呈线性。该测定法的重现性以及从血清和红细胞中回收阿莫地喹的情况均令人满意。该测定法的特异性以及诱导荧光团的性质尚不清楚。本文指出了5名受试者每千克体重给予10毫克阿莫地喹碱后，血清和红细胞中阿莫地喹的代表性水平。
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