利用色氨酸-乳糖操纵子融合菌株筛选大肠杆菌中的色氨酸调控突变体。
Isolating tryptophan regulatory mutants in Escherichia coli by using a trp-lac fusion strain.
作者信息
Reznikoff W S, Thornton K P
出版信息
J Bacteriol. 1972 Feb;109(2):526-32. doi: 10.1128/jb.109.2.526-532.1972.
Abstract
A trp-lac fusion strain of Escherichia coli in which the lac structural genes are part of the tryptophan operon has been used to isolate trp regulatory mutants. This was accomplished by isolating lac(+) colonies on either lactose-minimal agar or lactose-MacConkey indicator agar. Seventy-seven of 78 lac(+) isolates contained mutations which mapped near the ara locus and most of these isolates were found to be 5-methyltryptophan-resistant after introduction of an F-trp episome. The lac(+) phenotypes of these 77 isolates were therefore probably the result of trpR(-) mutations. The one remaining isolate carried a mutation which was not part of the trp regulatory system.
摘要
一种大肠杆菌的色氨酸-乳糖融合菌株，其中乳糖结构基因是色氨酸操纵子的一部分，已被用于分离色氨酸调节突变体。这是通过在乳糖基本琼脂或乳糖-麦康凯指示琼脂上分离lac(+)菌落来实现的。78个lac(+)分离株中有77个含有位于阿拉伯糖位点附近的突变，在引入F-色氨酸附加体后，发现这些分离株中的大多数对5-甲基色氨酸具有抗性。因此，这77个分离株的lac(+)表型可能是trpR(-)突变的结果。剩下的一个分离株携带的突变不属于色氨酸调节系统。
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本文引用的文献
1
Fusions of the lac and trp Regions of the Escherichia coli Chromosome.大肠杆菌染色体中乳糖操纵子和色氨酸操纵子区域的融合
J Bacteriol. 1970 Dec;104(3):1273-9. doi: 10.1128/jb.104.3.1273-1279.1970.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
[Inhibition of the synthesis of the enzymes participating in the formation of tryptophan in Escherichia coli].[对大肠杆菌中参与色氨酸形成的酶的合成的抑制作用]
C R Hebd Seances Acad Sci. 1959 Jun 15;248(24):3490-2.
4
Nonsense codons and polarity in the tryptophan operon.色氨酸操纵子中的无义密码子与极性
J Mol Biol. 1966 Nov 14;21(2):313-34. doi: 10.1016/0022-2836(66)90102-1.
5
Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.大肠杆菌乳糖操纵子区域的转位。I. 乳糖操纵子的倒位及噬菌体φ80介导的乳糖转导
J Mol Biol. 1966 Aug;19(2):254-65. doi: 10.1016/s0022-2836(66)80003-7.
6
Regulation of the enzymes of the tryptophan pathway in Escherichia coli.大肠杆菌中色氨酸途径中酶的调控
Genetics. 1965 Dec;52(6):1303-16. doi: 10.1093/genetics/52.6.1303.
7
Genetic mapping of the regulator and operator genes of the lac operon.乳糖操纵子调节基因和操纵基因的遗传定位。
J Mol Biol. 1968 Sep 28;36(3):413-7. doi: 10.1016/0022-2836(68)90165-4.
8
Operator mutants of the tryptophan operon in Escherichia coli.大肠杆菌中色氨酸操纵子的操纵基因突变体。
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